A few months ago, I wasn’t sure what to expect when looking at fluorescence microscopy images in published papers. I looked at the accompanying graph to understand the data or the point the authors were trying to make. Often, the graph represents one or more measures of the so-called co-localization, but I couldn’t figure out how to interpret them. It turned out; reading the images is simple. Cells are simultaneously stained by two dyes (say, red and green) for two different proteins.
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